Reconstruction of Hyper-Thermostable Ancestral L-Amino Acid Oxidase to Perform Deracemization to D-Amino Acids
Chiharu Ishida, Ryo Miyata, Fumihito Hasebe, Azusa Miyata, Shigenori Kumazawa, Sohei Ito, Shogo Nakano
ChemCatChem, in Press

Design of a full‐consensus glutamate decarboxylase and its application to GABA biosynthesis,
Hiroshi Takagi, Kohei Kozuka, Kenta Mimura, Shogo Nakano*, Sohei Ito
ChemBioChem, in Press

Discovery of a “Gatekeeper” Antagonist of Retinoid X Receptors without Allosteric Ligand Inhibition in Permissive RXR Heterodimers,
Masaki Watanabe, Michiko Fujihara, Tomoharu Motoyama, Mayu Kawasaki, Shoya Yamada, Yuta Takamura, Sohei Ito, Makoto Makishima, Shogo Nakano*,Hiroki Kakuta*,
Journal of Medicinal Chemistry, 64(1), 430-439 (2021)

Chemoenzymatic synthesis of 3-ethyl-2,5-dimethylpyrazine by L-threonine 3-dehydrogenase and 2-amino-3-ketobutyrate CoA ligase/L-threonine aldolase
Tomoharu Motoyama, Shogo Nakano*, Fumihito Hasebe, Ryo Miyata, Shigenori Kumazawa, Noriyuki Miyoshi and Sohei Ito*,
Communications Chemistry, 4, 108, (2021)

Catalytic Mechanism of Ancestral L-Lysine Oxidase Assigned by Sequence Data Mining,
Sayaka Sugiura1, Shogo Nakano1*, Masazumi Niwa, Fumihito Hasebe, Daisuke Matsui and Sohei Ito,
Journal of Biological Chemistry, 101043, doi: 10.1016/j.jbc.2021.101043, (2021)

Protein Sequence Selection Method That Enables Full Consensus Design of Artificial L-Threonine 3-Dehydrogenases With Unique Enzymatic Properties
Tomoharu Motoyama, Nozomi Hiramatsu, Yasuhisa Asano, Shogo Nakano*, Sohei Ito
Biochemistry, 2020, 59, 3823-3833

AirID, a novel proximity biotinylation enzyme, for analysis of protein-protein interactions.
Kido K, Yamanaka S, Nakano S, Motani K, Shinohara S, Nozawa A, Kosako H, Ito S, Sawasaki T.
eLife. 2020, 9, e54983

Following the Evolutionary Track of a Highly Specific L-Arginine Oxidase by Reconstruction and Biochemical Analysis of Ancestral and Native Enzymes
Nakano S, Niwa M, Asano Y, Ito S
Appl. Env. Microbiol., 2019, 85: e00459-19

Deracemization and Stereoinversion to Aromatic D-Amino Acid Derivatives with Ancestral L-Amino Acid Oxidase
Nakano S, Minamino Y, Hasebe F, Ito S
ACS Catalysis 2019, 9, 10152-10158

Benchmark Analysis of Native and Artificial NAD+-Dependent Enzymes Generated by a Sequence-Based Design Method with or without Phylogenetic Data
Nakano S*, Motoyama T, Miyashita Y, Ishizuka Y, Matsuo N, Tokiwa H, Shinoda S, Asano Y*, Ito S
Biochemistry, 2018, 57, 3722-3732