論文

研究業績一覧

当研究室に関する研究業績は伊藤創平(ORCID、Researchmap)および
旧スタッフ中野祥吾(ORCID、Researchmap)もご確認ください。

2024年

In vitro characterization of O-specific glycosyltransferase involved in flagellin glycosylation.
Daisuke Fujinami, Ayumi Mizui, Azusa Miyata and Sohei Ito
ACS Chemical Biology, in press
Structure Prediction and Genome Mining-Aided Discovery of the Bacterial C-Terminal Tryptophan Prenyltransferase PalQ
Azusa Miyata, Sohei Ito and Daisuke Fujinami
ADVANCED SCIENCE, 07 December 2023,
Design of Ancestral Sortase E that is Applicable in Protein Biomaterial Synthesis
Azusa Miyata, Taichi Chisuga, Akira Kambe, Ryo Miyata, Yui Kawamura, Hiroyuki Takeda, Sohei Ito and Shogo Nakano
ACS Catalysis, 2024, 14, 5, Pages3514–3523
Development of Evolutionary Algorithm-Based Protein Redesign Method
Hiroki Ozawa, Ibuki Unno, Ryohei Sekine, Taichi Chisuga, Sohei Ito and Shogo Nakano
Cell Reports Physical Science, 17 January 2024

2023年

Structural and functional analysis of hyper-thermostable ancestral L-amino acid oxidase that can convert Trp derivatives to D-forms by chemoenzymatic reaction
Yui Kawamura, Chiharu Ishida, Ryo Miyata, Azusa Miyata, Seiichiro Hayashi, Daisuke Fujinami, Sohei Ito and Shogo Nakano
Communications chemistry, 22 September 2023
Definition of an Index Parameter To Screen Highly Functional Enzymes Derived from a Biochemical and Thermodynamic Analysis of Ancestral meso-Diaminopimelate Dehydrogenases
Hayato Araseki, Mayu Kawasaki, Akira Kambe, Kohei Kozuka, Sohei Ito and Shogo Nakano
ChemBioChem, 03 July 2023

2022年

Enzymatic Conjugation of Modified RNA Fragments by Ancestral RNA Ligase AncT4_2
Shohei Kajimoto, Miwa Ohashi, Yusuke Hagiwara, Daisuke Takahashi, Yasuhiro Mihara, Tomoharu Motoyama, Sohei Ito and Shogo Nakano
Applied and Enviromental Microbiology, 23 Nov 2022
Reconstruction of Hyper-Thermostable Ancestral L-Amino Acid Oxidase to Perform Deracemization to D-Amino Acids
Chiharu Ishida, Ryo Miyata, Fumihito Hasebe, Azusa Miyata, Shigenori Kumazawa, Sohei Ito and Shogo Nakano
ChemCatChem, 15 December 2021, Pages 5228-5235
Design of a full‐consensus glutamate decarboxylase and its application to GABA biosynthesis
Hiroshi Takagi, Kohei Kozuka, Kenta Mimura, Shogo Nakano and Sohei Ito
ChemBioChem, 20 April, 2022

2021年

Discovery of a “Gatekeeper” Antagonist of Retinoid X Receptors without Allosteric Ligand Inhibition in Permissive RXR Heterodimers,
Masaki Watanabe, Michiko Fujihara, Tomoharu Motoyama, Mayu Kawasaki, Shoya Yamada, Yuta Takamura, Sohei Ito, Makoto Makishima, Shogo Nakano and Hiroki Kakuta
Journal of Medicinal Chemistry, 14 Jan 2021, Page 430-439
Chemoenzymatic synthesis of 3-ethyl-2,5-dimethylpyrazine by L-threonine 3-dehydrogenase and 2-amino-3-ketobutyrate CoA ligase/L-threonine aldolase
Tomoharu Motoyama, Shogo Nakano, Fumihito Hasebe, Ryo Miyata, Shigenori Kumazawa, Noriyuki Miyoshi and Sohei Ito
Communications Chemistry, 16 Jul 2021

2020年

Protein Sequence Selection Method That Enables Full Consensus Design of Artificial L-Threonine 3-Dehydrogenases With Unique Enzymatic Properties
Tomoharu Motoyama, Nozomi Hiramatsu, Yasuhisa Asano, Shogo Nakano and Sohei Ito
Biochemistry, 13 Oct 2020, Page 3823-3833
AirID, a novel proximity biotinylation enzyme, for analysis of protein-protein interactions.
Kohki Kido, Satoshi Yamanaka, Shogo Nakano, Kou Motani, Souta Shinohara, Akira Nozawa, Hidetaka Kosako, Sohei Ito and Tatsuya Sawasaki
eLife. 11 May 2020

2019年

Following the Evolutionary Track of a Highly Specific L-Arginine Oxidase by Reconstruction and Biochemical Analysis of Ancestral and Native Enzymes
Nakano S, Niwa M, Asano Y and Ito S
Applied and Enviromental Microbiology, 30 May 2019
Deracemization and Stereoinversion to Aromatic D-Amino Acid Derivatives with Ancestral L-Amino Acid Oxidase
Nakano S, Minamino Y, Hasebe F and Ito S
ACS Catalysis, 1 Nov 2019

2018年

Benchmark Analysis of Native and Artificial NAD+-Dependent Enzymes Generated by a Sequence-Based Design Method with or without Phylogenetic Data
Nakano S, Motoyama T, Miyashita Y, Ishizuka Y, Matsuo N, Tokiwa H, Shinoda S, Asano Y and Ito S
Biochemistry, 3 Jul 2018