論文

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2026年

A cyanobacterial adenine prenyltransferase enables longer-chain N6 prenylation
Kotoha Ichikawa, Kentaro Tamura, Koki Fujitani, Taichi Chisuga, Ryunosuke Takeda, Tomoki Sato, Seiichiro Hayashi, Koichi Kato, Shinji Miura, Shogo Nakano, Sohei Ito, Daisuke Fujinami
doi: https://doi.org/10.64898/2026.04.03.716432
Enzymatic Prenylation of Proteins and Peptides: From Cysteine S-Prenylation to Tryptophan-Selective Biocatalysis.
Daisuke Fujinami, Hikari Ozawa, Sohei Ito
Chemistry - A European Journal, in press
Structural and Biochemical Characterization of a Minimal Protein-Asparaginase
Takuto Ono, Hiroki Yamaguchi, Kazutoshi Takahashi, Yoshinori Hirao, Isao Abe, Taichi Chisuga, Daisuke Fujinami, Shogo Nakano, Sohei Ito
Chembiochem. 2026 Feb 25;27(4):e202500893.

2025年

Expanding the Chemical Space of Antimicrobial Peptides via Enzymatic Prenylation
Hikari Ozawa, Azusa Miyata, Seiichiro Hayashi, Noriyuki Miyoshi, Koichi Kato, Sohei Ito, Daisuke Fujinami
J. Am. Chem. Soc. 2025, 147, 29, 25642–25651
In Vitro Amyloid Formation by a Bacteriocin From Bifidobacterium longum subsp. infantis
Ayane Kumagai, Kouta Mayanagi, Seiichiro Hayashi, Shogo Nakano, Sohei Ito, Daisuke Fujinami
Proteins. 2025 Nov;93(11):1953-1961.
Ancestral sequence reconstruction as a tool for structural analysis of modular polyketide synthases
Taichi Chisuga, Shota Takinami, Zengwei Liao, Masayuki Karasawa, Naruhiko Adachi, Masato Kawasaki, Toshio Moriya, Toshiya Senda, Tohru Terada, Fumitaka Kudo, Tadashi Eguchi, Shogo Nakano, Sohei Ito & Akimasa Miyanaga
Nature Communications volume 16, Article number: 6847 (2025)
Arginine-N,N′-bisprenyltransferases: Switchable Catalysis in Consecutive Guanidine-N-prenylation
Kei Fujita, Yuito Yamada, Tomo Taniguchi, Daisuke Fujinami, Takahiro Mori, Kenichi Matsuda, Ikuro Abe, Toshiyuki Wakimoto
J. Am. Chem. Soc. 2025, 147, 28, 24766–24773

2024年

In vitro characterization of O-specific glycosyltransferase involved in flagellin glycosylation.
Daisuke Fujinami, Ayumi Mizui, Azusa Miyata and Sohei Ito
ACS Chem. Biol. 2024, 19, 4, 992–998
Structure Prediction and Genome Mining-Aided Discovery of the Bacterial C-Terminal Tryptophan Prenyltransferase PalQ
Azusa Miyata, Sohei Ito and Daisuke Fujinami
ADVANCED SCIENCE, 2024 Feb;11(6):e2307372
Design of Ancestral Sortase E that is Applicable in Protein Biomaterial Synthesis
Azusa Miyata, Taichi Chisuga, Akira Kambe, Ryo Miyata, Yui Kawamura, Hiroyuki Takeda, Sohei Ito and Shogo Nakano
ACS Catalysis, 2024, 14, 5, Pages3514–3523
Development of Evolutionary Algorithm-Based Protein Redesign Method
Hiroki Ozawa, Ibuki Unno, Ryohei Sekine, Taichi Chisuga, Sohei Ito and Shogo Nakano
Cell Reports Physical Science, 17 January 2024

2023年

Structural and functional analysis of hyper-thermostable ancestral L-amino acid oxidase that can convert Trp derivatives to D-forms by chemoenzymatic reaction
Yui Kawamura, Chiharu Ishida, Ryo Miyata, Azusa Miyata, Seiichiro Hayashi, Daisuke Fujinami, Sohei Ito and Shogo Nakano
Communications chemistry, 22 September 2023
Definition of an Index Parameter To Screen Highly Functional Enzymes Derived from a Biochemical and Thermodynamic Analysis of Ancestral meso-Diaminopimelate Dehydrogenases
Hayato Araseki, Mayu Kawasaki, Akira Kambe, Kohei Kozuka, Sohei Ito and Shogo Nakano
ChemBioChem, 03 July 2023
Residue-based correlation between equilibrium and rate constants is an experimental formulation of the consistency principle for smooth structural changes of proteins
Daisuke Kohda, Seiichiro Hayashi, Daisuke Fujinami
Biophys Physicobiol. 2023 Dec 13;20(4):e200046

2022年

Enzymatic Conjugation of Modified RNA Fragments by Ancestral RNA Ligase AncT4_2
Shohei Kajimoto, Miwa Ohashi, Yusuke Hagiwara, Daisuke Takahashi, Yasuhiro Mihara, Tomoharu Motoyama, Sohei Ito and Shogo Nakano
Applied and Enviromental Microbiology, 23 Nov 2022
Reconstruction of Hyper-Thermostable Ancestral L-Amino Acid Oxidase to Perform Deracemization to D-Amino Acids
Chiharu Ishida, Ryo Miyata, Fumihito Hasebe, Azusa Miyata, Shigenori Kumazawa, Sohei Ito and Shogo Nakano
ChemCatChem, 15 December 2021, Pages 5228-5235
Design of a full‐consensus glutamate decarboxylase and its application to GABA biosynthesis
Hiroshi Takagi, Kohei Kozuka, Kenta Mimura, Shogo Nakano and Sohei Ito
ChemBioChem, 20 April, 2022

2021年

Discovery of a “Gatekeeper” Antagonist of Retinoid X Receptors without Allosteric Ligand Inhibition in Permissive RXR Heterodimers,
Masaki Watanabe, Michiko Fujihara, Tomoharu Motoyama, Mayu Kawasaki, Shoya Yamada, Yuta Takamura, Sohei Ito, Makoto Makishima, Shogo Nakano and Hiroki Kakuta
Journal of Medicinal Chemistry, 14 Jan 2021, Page 430-439
Chemoenzymatic synthesis of 3-ethyl-2,5-dimethylpyrazine by L-threonine 3-dehydrogenase and 2-amino-3-ketobutyrate CoA ligase/L-threonine aldolase
Tomoharu Motoyama, Shogo Nakano, Fumihito Hasebe, Ryo Miyata, Shigenori Kumazawa, Noriyuki Miyoshi and Sohei Ito
Communications Chemistry, 16 Jul 2021

2020年

Protein Sequence Selection Method That Enables Full Consensus Design of Artificial L-Threonine 3-Dehydrogenases With Unique Enzymatic Properties
Tomoharu Motoyama, Nozomi Hiramatsu, Yasuhisa Asano, Shogo Nakano and Sohei Ito
Biochemistry, 13 Oct 2020, Page 3823-3833
AirID, a novel proximity biotinylation enzyme, for analysis of protein-protein interactions.
Kohki Kido, Satoshi Yamanaka, Shogo Nakano, Kou Motani, Souta Shinohara, Akira Nozawa, Hidetaka Kosako, Sohei Ito and Tatsuya Sawasaki
eLife. 11 May 2020

2019年

Following the Evolutionary Track of a Highly Specific L-Arginine Oxidase by Reconstruction and Biochemical Analysis of Ancestral and Native Enzymes
Nakano S, Niwa M, Asano Y and Ito S
Applied and Enviromental Microbiology, 30 May 2019
Deracemization and Stereoinversion to Aromatic D-Amino Acid Derivatives with Ancestral L-Amino Acid Oxidase
Nakano S, Minamino Y, Hasebe F and Ito S
ACS Catalysis, 1 Nov 2019

2018年

Benchmark Analysis of Native and Artificial NAD+-Dependent Enzymes Generated by a Sequence-Based Design Method with or without Phylogenetic Data
Nakano S, Motoyama T, Miyashita Y, Ishizuka Y, Matsuo N, Tokiwa H, Shinoda S, Asano Y and Ito S
Biochemistry, 3 Jul 2018